LST-6300
Saliva Alcohol Screening Test

Procedure:
1. Open the foil package and remove the test strip.
2. Saturate the reactive pad by dipping the reaction pad into the saliva specimen collected in a sputum cup, or by applying saliva directly to the reaction pad. After 10 seconds, shake off the excess saliva.
3. Immediately start timer and at 2 minutes, compare the reactive pad with the provided colored chart.
Results after more than 2 minutes may be not accurate.

Materials Provided:
1. Main materials:
Tetramethylbenzidine (TMB) ---------0.12mg
Alcohol oxidase (EC)------------------0.5 IU
Peroxidase (EC)-----------------------35 IU
Proteins ---------------------------------0.15mg
2. Auxiliary material: PVC
3. Package material: foil

Precautions:
1. For in vitro use only.
2. Do not use the product beyond expiration date.
3. Handle all specimens as potentially infectious.
4. The product is sensitive to the presence of alcohol and moisture. After open the package, the test device should be used immediately.

Specimen Collection and Preparation:
1. Nothing should be placed into the mouth of the subject for at least 10 minutes prior to saliva collection. This includes food, drink, tobacco products or other materials.
2. Saliva specimen can be collected in a sputum cup or a clean container, or directly applied to the reaction pad of the test strip.
3. Avoid contact with skin by wearing gloves and proper laboratory attire.

Interpretation of Results:
Negative: Almost no color change by comparing with the background. The negative result indicates that the BAC is less than 0.02%.
Positive: A distinct color developed all over the pad. The positive result indicates that the BAC is 0.02% or higher.
Invalid: The test should be considered invalid If only the edge of the reactive pad turned color that might be ascribed to insufficient sampling. The subject should be re-tested.